.:: DNA SOPs

	Services SOPs available EuroBioBank partner Country Name
	Manual DNA extraction from blood or lymphocytes with phenol/chloroform Yes France, Malta, Italy Généthon, UOM, SUN, NMTB DNA extraction from blood through salting out procedure Yes France, Hungary Généthon, NCPH Autogen® automated DNA extraction from blood or lymphocytes with phenol/chloroform Yes France Généthon DNA extraction from lymphocytes Yes France Généthon DNA extraction from muscle Yes Italy IRCCS

.:: Tissue SOPs

	Services SOPs available EuroBioBank partner Country Name
	Freezing and thawing of vital muscle biopsy for culture Yes Italy INNCB Isopentane freezing protocol for muscle biopsy Yes France AFM Nitrogen freezing protocol for muscle biopsy Yes France AFM

.:: Common aspects for human cell culture

	Services SOPs available EuroBioBank partner Country Name
	Good practices for cell culture techniques (Aseptic and Safety aspects of cell culture) Yes Italy INNCB Sterilization and filtration of media and reagents-Serum thawing, heat inactivation and testing Yes Italy INNCB Freezing, cryopreservation, storage and reactivation of cell lines Yes Italy INNCB Detection of contaminants in human cell culture Yes Italy INNCB; EBB Routine cell counting and assessment of viability Yes Italy INNCB

.:: Myoblast cell culture

	Services SOPs available EuroBioBank partner Country Name
	Primary myoblast culture from fresh human muscle biopsy Yes Italy INNCB Primary myoblast culture from fresh or frozen human muscle biopsy Yes Germany MTCC Myoblast purity assessment by FACS Yes Italy INNCB Myoblast purity assessment by microbeads Yes Italy INNCB Myoblast immortalisation Yes Germany MTCC Muscle cell innervation with foetal rat spinal cord Yes Italy, France INNCB, NMTB, BET Collection of innervated myotubes using the sandwich technique Yes Italy INNCB, NMTB

.:: Fibroblast cell culture

	Services SOPs available EuroBioBank partner Country Name
	Primary fibroblast culture from human skin biopsy Yes Italy INNCB

.:: Lymphocyte and suspension cell line culture

	Services SOPs available EuroBioBank partner Country Name
	Purification of lymphocytes from peripheral blood Yes France Généthon Subculture of suspension cell lines Yes Italy INNCB EBV immortalization of lymphocytes Yes Italy INNCB

.:: Packaging and transport of biological resources

	Services SOPs available EuroBioBank partner Country Name
	Protocol for the conditioning and transport of biomaterials Yes France AFM Protocol for transport of tissue samples Yes France, Italy AFM, INNCB Protocol for transporting cells at room temperature or frozen Yes France AFM Safety instructions in the event of an accident Yes France AFM European directives for transport of biological material Yes France AFM