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DNA | Tissues | Common aspects for human cell culture | Myoblast | Fibroblast | Lymphocyte | Packaging

.:: DNA SOPs

Services EuroBioBank Partners/Authors
Manual DNA extraction from blood or lymphocytes with phenol/chloroform Généthon – S.Saker, UOM – A.Felice, SUN – L.Politano, NMTB –E.Pegoraro
Manual DNA extraction from blood through salting out Généthon – S.Saker, NCPH –V.Karcagi
Autogen® automated DNA extraction from blood or lymphocytes with phenol/chloroform Généthon – S.Saker
DNA extraction from muscle NMUNIT–UNIMIOM – M.Moggio
DNA extraction from lymphocytes Généthon – S.Saker

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.:: Tissue SOPs

Services EuroBioBank Partners/Authors
Isopentane freezing protocol for muscle biopsy AFM – M.Chapart
Nitrogen freezing protocol for muscle biopsy AFM – M.Chapart

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.:: Common aspects for human cell culture

Services EuroBioBank Partners/Authors
Good practices for cell culture techniques (Aseptic and Safety aspects of cell culture) NEUMD – INNCB–M.Mora
Sterilization and filtration of media and reagents-Serum thawing, heat inactivation and testing NEUMD – INNCB–M.Mora
Freezing, cryopreservation, storage and reactivation of cell lines NEUMD – INNCB–M.Mora
Detection of contaminants in human cell culture NEUMD – INNCB–M.Mora
Routine cell counting and assessment of viability NEUMD – INNCB–M.Mora

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.:: Myoblast cell culture

Services EuroBioBank Partners/Authors
Primary myoblast culture from fresh human muscle biopsy, cultured as explants NEUMD – INNCB–M.Mora
Primary myoblast culture from muscle biopsy, cultured after enzyme dissociation MTCC – P.Schneiderat
Myoblast purity assessment by FACS NEUMD – INNCB–M.Mora
Myoblast purity assessment by microbeads NEUMD – INNCB–M.Mora
Myoblast immortalisation MTCC – P. Schneiderat
Muscle cell innervation with foetal rat spinal cord NEUMD – INNCB–M.Mora, NMTB – C. Angelini
Collection of innervated myotubes using the sandwich technique NEUMD – INNCB–M.Mora

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.:: Lymphocyte and suspension cell line culture

Services EuroBioBank Partners/Authors
Primary fibroblast culture from human skin biopsy NEUMD–NNCB – M.Mora, MDB–INNCB – B.Garavaglia
Purification of lymphocytes from peripheral blood Généthon–S.Saker
Subculture of suspension cell lines NEUMD – INNCB–M.Mora
EBV immortalization of lymphocytes NEUMD – INNCB–M.Mora

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.:: Packaging and transport of biological resources

Services EuroBioBank Partners/Authors
Protocol for the conditioning and transport of biomaterials Généthon–S.Saker
Protocol for transport of tissue samples AFM–M.Chapart
Protocol for transporting cells at room temperature or frozen NEUMD – INNCB–M.Mora
Safety instructions in the event of an accident AFM – M.Chapart
European directives for transport of biological material NEUMD – INNCB–M.Mora


Last updated January 2015

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